BBa_K1363004BBa_K1363004 Version 1 (Component)the downstream passway of the blue light system which can be transfered into Caulobacter crescentus
BBa_J23058BBa_J23058 Version 1 (Component)[GFPmut3] inserted into [key3d]
BBa_I741008BBa_I741008 Version 1 (Component)brings xylose into the cell (doesn
pSB3K1pSB3K1 Version 1 (Component)pACYC177 converted into BioBrick vector (formerly BBa_I739202)
pSB4C1pSB4C1 Version 1 (Component)pCK01 converted into BioBrick vector (formerly BBa_I739202)
BBa_K283021BBa_K283021 Version 1 (Component)pTet-rbs-cheZ-termintors
BBa_J70572BBa_J70572 Version 1 (Component)J70570(AK) +J70568(AT) into (AC)
BBa_K563054BBa_K563054 Version 1 (Component)Vector pYD, and can transfer into nucles of S.cerevisivae
pSBBs4SBBa_K823022 Version 1 (Component)pSB<sub>Bs</sub>4S: Empty backbone for integration into <i/>Bacillus subtilis thrC</i> locus
pSBBs1CBBa_K823023 Version 1 (Component)pSB<sub>Bs</sub>1C: Empty backbone for integration into <i>Bacillus subtilis</i> <i>amyE</i> locus
pSBBs2EBBa_K823027 Version 1 (Component)pSB<sub>Bs</sub>2E: Empty backbone for integration into Bacillus subtilis lacA locus
BBa_J100110BBa_J100110 Version 1 (Component)P5 promoter cloned into scaffold
BBa_J100111BBa_J100111 Version 1 (Component)eCDM8 and Tetracycline Resistance Riboswitch into pSB1A8
BBa_J100113BBa_J100113 Version 1 (Component)sfGFP cloned into J100112
BBa_J100112BBa_J100112 Version 1 (Component)BD24 C.dog cloned into J100110
BBa_J100114BBa_J100114 Version 1 (Component)eCDM8 and GFP Riboswitch into pSB1A8
BBa_J100115BBa_J100115 Version 1 (Component)eCDM8 and GFP Riboswitch into pSB1A8
BBa_J100116BBa_J100116 Version 1 (Component)AdhE gene cloned into J100112
BBa_K1773026BBa_K1773026 Version 1 (Component)IPTG inducible cI repressor cloned with LuxI gene
BBa_K1655001BBa_K1655001 Version 1 (Component)This GFP can be fused into any protein's aminoterminal end with the BioBrick enzyme assembly method.
alkJBBa_K826001 Version 1 (Component)converts aliphatic medium-chain-length alcohols into aldehydes
BBa_K1085014BBa_K1085014 Version 1 (Component)pSB1AC3-HySp: Integrational backbone into <i>B. subtilis amyE</i> locus with IPTG inducible promoter
BBa_K1124117BBa_K1124117 Version 1 (Component)anti-csrA sRNA (+termintor) (sRNA generator)
BBa_K1124116BBa_K1124116 Version 1 (Component)anti-tyrR sRNA (+termintor) (sRNA generator)
BBa_K1124115BBa_K1124115 Version 1 (Component)anti-hycA sRNA (+termintor) (sRNA generator)
BBa_K1124114BBa_K1124114 Version 1 (Component)anti-luxI sRNA (+termintor) (sRNA generator)
BBa_K1124113BBa_K1124113 Version 1 (Component)anti-mCherry sRNA (+termintor) (sRNA generator)
BBa_K1217008BBa_K1217008 Version 1 (Component)Localizing polyphosphate kinase (ppk1) into native Eut microcompartment (BBa_K311004)
BBa_K1217009BBa_K1217009 Version 1 (Component)Localizing polyphosphate kinase (ppk1) into Histag modified Eut microcompartment (BBa_K311004)
BBa_K1217010BBa_K1217010 Version 1 (Component)Localizing PPK1 into Simple Eut Microcompartment (encoded by EutS gene only)
BBa_K1113600BBa_K1113600 Version 1 (Component)GFP with a targeting sequence for delivery into the Carboxysome and a ssrA degradation tag.
BBa_K1113501BBa_K1113501 Version 1 (Component)GFP with a targeting sequence for delivery into the Carboxysome and a ssrA degradation tag
BBa_K1113502BBa_K1113502 Version 1 (Component)GFP with a targeting sequence for delivery into the Carboxysome and a ssrA degradation tag
BBa_K1404001BBa_K1404001 Version 1 (Component)Ptac-NiCoTB-rcn-csgBAEFG, turns E. coli into a functional Ni and Co biofilter
BBa_K1363003BBa_K1363003 Version 1 (Component)OriT-K592016 the blue sensor which can be transfered into other bacterias by S17-1
BBa_K1329002BBa_K1329002 Version 1 (Component)Insertion of the Metallothionein Cup-1-1 domain into Bacillus subtilis flagellin
BBa_K1431201BBa_K1431201 Version 1 (Component)NLS, lead protein into the nucleus
BBa_K1937002BBa_K1937002 Version 1 (Component)OriKan: a composite part to turn any pSB1C3 into a <i>Bacillus</i> plasmid
DigitalizerDigitalizer_collection Version 1 (Collection)A genetic device to digitalize gene expression into a sharp on/off signal.
BBa_K581008BBa_K581008 Version 1 (Component)RBS+ccdB+Termintor
Intein_assisted_Bisection_MappingIntein_assisted_Bisection_Mapping_collection Version 1 (Collection)Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.
BBa_K112245BBa_K112245 Version 1 (Component){pAH57 promoter}{rbs.xis.int!} inserted into K112995 plasmid
BBa_K112246BBa_K112246 Version 1 (Component){pAH57 promoter}{rbs.xis.int!}{rbs.ihfA!}{rbs.ihfB!} inserted into K112995 plasmid
BBa_K112247BBa_K112247 Version 1 (Component){pAH57 promoter}{rbs.xis.int!} inserted into K112950 plasmid
BBa_K112248BBa_K112248 Version 1 (Component){pAH57 promoter}{rbs.xis.int!}{rbs.ihfA!}{rbs.ihfB!} inserted into K112950 plasmid
BBa_K146001BBa_K146001 Version 1 (Component)AOX1 ethanol sensitive promoter cloned into RFP vector BBa_J61002
BBa_K223035BBa_K223035 Version 1 (Component)BLH gene- cleaves beta-carotene into retinoic acid